TS-045681 — Cas9-derived constructs can be used to modulate gene expression, but physical limitations restrict the application of this technique. Researchers at The Ohio State University have overcome this obstacle by engineering new Cas9-derived transcription factors.
CRISPR/Cas9 systems have revolutionized the field of programmable DNA technology. Recently, a version of the Cas9 protein with a deactivated nuclease domain called “dCas9” was developed. Without this domain, dCas9’s range of activity is limited to recognizing and binding sequence…
